期刊
JOURNAL OF BIOMECHANICS
卷 40, 期 4, 页码 786-794出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2006.03.013
关键词
cell; mechanics; nucleus; strain; extracellular matrix; intervertebral disc
资金
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR047442, P01AR050245, R01AR048182] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM008555] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG015768] Funding Source: NIH RePORTER
- NIAMS NIH HHS [AR47442, AR50245, AR48182] Funding Source: Medline
- NIA NIH HHS [AG15768] Funding Source: Medline
- NIGMS NIH HHS [T32-GM08555] Funding Source: Medline
Mechanical stimuli are important factors that regulate cell proliferation, survival, metabolism and motility in a variety of cell types. The relationship between mechanical deformation of the extracellular matrix and intracellular deformation of cellular sub-regions and organelles has not been fully elucidated, but may provide new insight into the mechanisms involved in transducing mechanical stimuli to biological responses. In this study, a novel fluorescence microscopy and image analysis method was applied to examine the hypothesis that mechanical strains are fully transferred from a planar, deformable substrate to cytoplasmic and intranuclear regions within attached cells. Intracellular strains were measured in cells derived from the anulus fibrosus of the intervertebral disc when attached to an elastic silicone membrane that was subjected to tensile stretch. Measurements indicated cytoplasmic strains were similar to those of the underlying substrate, with a strain transfer ratio (STR) of 0.79. In contrast, nuclear strains were much smaller than those of the substrate, with an STR of 0.17. These findings are consistent with previous studies indicating nuclear stiffness is significantly greater than cytoplasmic stiffness, as measured using other methods. This study provides a novel method for the study of cellular mechanics, including a new technique for measuring intranuclear deformations, with evidence of differential magnitudes and patterns of strain transferred from the substrate to cell cytoplasm and nucleus. (c) 2006 Elsevier Ltd. All rights reserved.
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