期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 21, 期 7, 页码 1150-1154出版社
WILEY
DOI: 10.1002/rcm.2947
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资金
- NCI NIH HHS [R33 CA105295] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R33CA105295] Funding Source: NIH RePORTER
We report the characterization of a recently introduced hybrid ionization source, matrix-assisted laser desorption electrospray ionization (MALDESI), coupled to a quadrupole Fourier transform ion cyclotron resonance mass spectrometry (QFT-ICR-MS) system. We first demonstrate the ability of MALDESI-QFT-ICR MS to directly analyze and provide high mass measurement accuracy (similar to 1 part-per-million) of a polypeptide using internal calibration. Second, we show the potential of MALDESI-QFT-ICR MS for the top-down characterization of multiply charged polypeptide cations. Finally, we demonstrate sub-femtomole detection limits in MALDESI-QFT-ICR MS using a combination of naturally occurring peptides and their respective stable isotope labeled forms. The results presented herein demonstrate the feasibility of several potential applications for MALDESI-QFT-CR MS for the direct analysis of intact biological molecules. Copyright (c) 2007 John Wiley & Sons, Ltd.
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