期刊
JOURNAL OF CELL BIOLOGY
卷 176, 期 1, 页码 27-33出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200609116
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- NIBIB NIH HHS [R01 EB001480] Funding Source: Medline
- NIGMS NIH HHS [R01 GM35227] Funding Source: Medline
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB001480] Funding Source: NIH RePORTER
Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin-Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin 11 regulatory light chain accumulated at two locations: (I) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin 11 activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring.
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