期刊
JOURNAL OF PROTEOME RESEARCH
卷 6, 期 6, 页码 2152-2160出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr060580k
关键词
acquired enamel pellicle; proteomics; oral; mass spectrometry; LC-ESI-MS/MS; linear ion trap; proteins; saliva
资金
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE007652] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [U01DE014950, R01DE005672, R37DE005672] Funding Source: NIH RePORTER
- NIDCR NIH HHS [DE07652, DE14950, DE05672] Funding Source: Medline
The acquired enamel pellicle is a thin protein film forming upon exposure of tooth enamel surfaces to saliva. The structural analysis of this integument relies on efficient pellicle harvesting and protein identification procedures. Material from three individual subjects and two pooled samples yielded the identification by LC-ESI-MS/MS of 130 pellicle proteins of which 89 were found in three or more experiments. A high intersubject consistency in pellicle composition was observed.
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