期刊
JOURNAL OF PROTEOME RESEARCH
卷 6, 期 6, 页码 2205-2210出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr0607031
关键词
electrospray-ionization mass spectrometry (ESI-MS); Fourier-transform mass spectrometry (FT-MS); Fourier-transform ion cyclotron resonance (FTICR); collision activated dissociation (CAD); electron capture dissociation (ECD); activated-ion ECD (aiECD); infrared multiphoton dissociation (IRMPD); thylakoid membrane; membrane protein; ATP synthase
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [P01HL080111] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI067769] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [U01DE016275] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [P01NS049134] Funding Source: NIH RePORTER
- NHLBI NIH HHS [P01 HL80111-01] Funding Source: Medline
- NIAID NIH HHS [1U19 AI067769] Funding Source: Medline
- NIDCR NIH HHS [U01 DE016275-01] Funding Source: Medline
- NINDS NIH HHS [P01 NS049134-01] Funding Source: Medline
The c-subunit of ATP synthase (AtpH) is an 8 kD integral membrane protein with two transmembrane domains; we set out to demonstrate it amenable to top-down electrospray-ionization Fourier-transform mass spectrometry ( FT-MS) using both collision activated and electron capture dissociation ( CAD/ECD). Thermal activation concomitant with electron delivery was necessary for efficient ECD ( activated-ion ECD; aiECD), yielding complementary information and greater sequence coverage in the transmembrane domains in comparison with CAD.
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