期刊
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
卷 175, 期 1, 页码 45-54出版社
AMER THORACIC SOC
DOI: 10.1164/rccm.200601-062OC
关键词
familial interstitial pneumonia; global transcription analysis; interstitial lung disease; lung fibrosis; microarrays
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [U01HL067467] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [Z01ES102546, P30ES011961, U19ES011375, Z01ES101947] Funding Source: NIH RePORTER
- Intramural NIH HHS Funding Source: Medline
- NHLBI NIH HHS [HL67467] Funding Source: Medline
- NIEHS NIH HHS [ES011961, ES11375] Funding Source: Medline
Rationale: Idiopathic interstitial pneumonia (IIP) and its familial variants are progressive and largely untreatable disorders with poorly understood molecular mechanisms. Both the genetics and the histologic type of IIP play a role in the etiology and pathogenesis of interstitial lung disease, but transcriptional signatures of these sub-types are unknown. Objectives: To evaluate gene expression in the lung tissue of patients with usual interstitial pneumonia or nonspecific interstitial pneumonia that was either familial or nonfamilial in origin, and to compare it with gene expression in normal lung parenchyma. Methods: We profiled RNA from the lungs of 16 patients with sporadic IIP, 10 with familial HIP, and 9 normal control subjects on a whole human genome oligonucleotide microarray. Results: Significant transcriptional differences exist in familial and sporadic lips. The genes distinguishing the genetic subtypes belong to the same functional categories as transcripts that distinguish lip from normal samples. Relevant categories include chemokines and growth factors and their receptors, complement components, genes associated with cell proliferation and death, and genes in the Wnt pathway. The role of the chemokine CXCL12 in disease pathogenesis was confirmed in the murine bleomycin model of lung injury, with C57BL/6(CXCR4+/-) mice demonstrating significantly less Collagen deposition than C57BL/(6XCR4+/+) mice. Whereas substantial differences exist between familial and sporadic IIPs, we identified only minor gene expression changes between usual interstitial pneumonia and nonspecific interstitial pneumonia . Conclusions: Taken together, our findings indicate that differences in gene expression profiles between familial and sporadic UPS may provide clues to the etiology and pathogenesis of IIP.
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