期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 71, 期 1, 页码 279-282出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.60647
关键词
molecular chaperone; DnaK; DnaJ; RNase E; psbA
Three dnaK and four dnaJ genes have been identified in the genome of cyanobacterium Syneehoeoceus elongatus PCC 7942. Our comprehensive analysis of yeast two-hybrid screening revealed a specific interaction among DnaK2, DnaJ2, and RNase E, an essential endoribonuclease. We examined the effects of DnaK2 and DnaJ2 on RNase E activity by monitoring the digestion of psbAII transcript in vitro. The addition of DnaK2 and DnaJ2 obviously inhibited RNase E activity in an ATP-dependent manner. These results suggest that DnaK2 and DnaJ2 are involved in RNA degradation through interaction with RNase E.
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