期刊
STEM CELLS
卷 25, 期 9, 页码 2245-2256出版社
WILEY
DOI: 10.1634/stemcells.2007-0128
关键词
adult bone marrow stein cells; bone marrow transplantation; in vitro differentiation; irradiation; microvesicles
资金
- NCRR NIH HHS [P20RR018757-04, P20 RR018757, P20 RR018757-047434] Funding Source: Medline
- NHLBI NIH HHS [K08 HL072332, 1K08 HL072332-01, 1R01HL73747-02] Funding Source: Medline
- NIDDK NIH HHS [1R01DK61858-03, 5K08 DK064980, R01 DK061858, K08 DK064980] Funding Source: Medline
- NIGMS NIH HHS [R01 GM047368, R01 GM047368-06A2, R01 GM047368-07, GM47368] Funding Source: Medline
- NINDS NIH HHS [R01 NS046810] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR018757] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [K08HL072332] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [K08DK064980, R01DK061858] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM047368] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS046810] Funding Source: NIH RePORTER
Numerous animal studies have demonstrated that adult marrow-derived cells can contribute to the cellular component of the lung. Lung injury is a major variable in this process; however, the mechanism remains unknown. We hypothesize that injured lung is capable of inducing epigenetic modifications of marrow cells, influencing them to assume phenotypic characteristics of lung cells. We report that under certain conditions, radiation-injured lung induced expression of pulmonary epithelial cell-specific genes and prosurfactant B protein in cocultured whole bone marrow cells separated by a cell-impermeable membrane. Lung-conditioned media had a similar effect on cocultured whole bone marrow cells and was found to contain pulmonary epithelial cell-specific RNA-filled microvesicles that entered whole bone marrow cells in culture. Also, whole bone marrow cells cocultured with lung had a greater propensity to produce type II pneumocytes after transplantation into irradiated mice. These findings demonstrate alterations of marrow cell phenotype by lung-derived microvesicles and suggest a novel mechanism for marrow cell-directed repair of injured tissue.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据