期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 170, 期 3, 页码 921-929出版社
AMER SOC INVESTIGATIVE PATHOLOGY, INC
DOI: 10.2353/ajpath.2007.060664
关键词
-
类别
Lactadherin is a secreted extraceffular matrix protein expressed in phagocytes and contributes to the removal of apoptotic cells. We examined lactadherin expression in brain sections of patients with or without Alzheimer's disease and studied its role in the phagocytosis of amyloid beta-peptide (A beta). Cells involved in Alzheimer's disease, including vascular smooth muscle cells, astrocytes, and microglia, showed a time-related increase in lactadherin production in culture. Quantitative analysis of the level of lactadherin showed a 35% reduction in lactadherin mRNA expression in the brains of patients with Alzheimer's disease (n = 52) compared with age-matched controls (n = 58; P = 0.003). Interestingly, lactadherin protein was detected in the brains of patients with Alzheimer's disease and controls, with low expression in areas rich in senile plaques and marked expression in areas without A beta deposition. Using surface plasmon resonance, we observed a direct protein-protein interaction between recombinant lactadherin and A beta 1-42 peptide in vitro. Lactadherin deficiency or its neutralization using specific antibodies significantly prevented A beta 1-42 phagocytosis by murine and human macrophages. in conclusion, lactadherin plays an important role in the phagocytosis of A beta 1-42 peptide, and its expression is reduced in Alzheimer's disease. Alterations in lactadherin production/function may contribute to the initiation and/or progression of Alzheimer's disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据