期刊
JOURNAL OF NEUROTRAUMA
卷 24, 期 1, 页码 43-53出版社
MARY ANN LIEBERT INC
DOI: 10.1089/neu.2006.0061
关键词
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资金
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [T32HD040686] Funding Source: NIH RePORTER
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [K23HD043843] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR000005] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [P50NS030318, P20NS030318, R01NS038087, P01NS030318, R01NS042648] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON DRUG ABUSE [U54DA021519] Funding Source: NIH RePORTER
- NCATS NIH HHS [UL1 TR000005] Funding Source: Medline
- NICHD NIH HHS [T32 HD040686, K23 HD043843, K23 HD 043843-01, HD 40686] Funding Source: Medline
- NIDA NIH HHS [U54 DA021519, U54 DA 021519] Funding Source: Medline
- NINDS NIH HHS [P50 NS030318, NS 42648, NS 30318, P01 NS030318, R01 NS042648, NS 38087, R01 NS042648-04, R01 NS038087] Funding Source: Medline
Traumatic brain injury (TBI) is the most common cause of traumatic death in infancy, and inflicted TBI (iTBI) is the predominant cause. Like other central nervous system pathologies, TBI changes the composition of cerebrospinal fluid (CSF), which may represent a unique clinical window on brain pathophysiology. Proteomic analysis, including two-dimensional (2-D) difference in gel electrophoresis (DIGE) combined with mass spectrometry (MS), was used to compare the CSF protein profile of two pooled samples from pediatric iTBI (n = 13) and non-inflicted TBI (nTBI; n = 13) patients with severe injury. CSF proteins from iTBI and nTBI were fluorescently labeled in triplicate using different fluorescent Cy dyes and separated by 2-D gel electrophoresis. Approximately 250 protein spots were found in CSF, with 90% between-gel reproducibility of the 2-D gel. Following in-gel digestion, the tryptic peptides were analyzed by MS for protein identification. The acute phase reactant, haptoglobin (HP) isoforms, showed an approximate fourfold increase in nTBI versus iTBI. In contrast, the levels of prostaglandin D-2 synthase (PGDS) and cystatin C (CC) were 12-fold and sevenfold higher in iTBI versus nTBI, respectively. The changes of HP, PGDS, and CC were confirmed by Western blot. These initial results with conventional gel-based proteomics show new protein changes that may ultimately help to understand pathophysiological differences between iTBI and nTBI.
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