期刊
NATURE PROTOCOLS
卷 2, 期 6, 页码 1307-1316出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2007.178
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资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM064426] Funding Source: NIH RePORTER
- NHGRI NIH HHS [HG00022] Funding Source: Medline
- NIGMS NIH HHS [GM64426] Funding Source: Medline
Dictyostelium discoideum, a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.
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