期刊
AIDS RESEARCH AND HUMAN RETROVIRUSES
卷 23, 期 1, 页码 86-92出版社
MARY ANN LIEBERT INC
DOI: 10.1089/aid.2006.0129
关键词
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Quantitative analysis of cell-mediated immune responses induced by candidate HIV vaccines requires robust procedures for collecting and processing human peripheral mononuclear blood cells (PBMCs). We evaluated several parameters in order to optimize a sample handling process that would be suitable for a multicenter clinical trial. Among the findings, systematic increases in the magnitude of IFN-gamma ELISpot responses were observed when the time from blood collection to PBMC freezing was reduced to < 12 h. By implementing these improvements within an ongoing clinical trial, the estimated immunologic response rates to an adenovirus-based HIV vaccine increased by more than 20 percentage points to similar to 80% of the vaccine recipients against any of the vaccine antigens and the average levels of T cell response improved more than 3-fold. These studies establish the importance of optimal conditions for PBMC collection and handling to the success of a clinical development program.
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