期刊
NATURE PROTOCOLS
卷 2, 期 1, 页码 221-226出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2006.375
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资金
- NCI NIH HHS [CA77839] Funding Source: Medline
- NIDDK NIH HHS [DK48831] Funding Source: Medline
- NIEHS NIH HHS [ES13125] Funding Source: Medline
- NIGMS NIH HHS [GM154312] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [P01CA077839] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK048831] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P01ES013125] Funding Source: NIH RePORTER
Oxidant stress has been implicated in a wide variety of disease processes. One method to quantify oxidative injury is to measure lipid peroxidation. Quantification of a group of prostaglandin F-2 alpha-like compounds derived from the nonezymatic oxidation of arachidonic acid, termed the F-2-isoprostanes (F-2-IsoPs), provides an accurate assessment of oxidative stress both in vitro and in vivo. In fact, in a recent independent study sponsored by the National Institutes of Health (NIH), F-2-IsoPs were shown to be the most reliable index of in vivo oxidant stress when compared against other well known biomarkers. This protocol details our laboratory's method to quantify F-2-IsoPs in biological fluids and tissues using gas chromatography-mass spectrometry (GC-MS). This procedure can be completed for 12-15 samples in 6-8 h.
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