4.7 Article

Immobilization of enzymes on heterofunctional epoxy supports

期刊

NATURE PROTOCOLS
卷 2, 期 5, 页码 1022-1033

出版社

NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2007.133

关键词

-

向作者/读者索取更多资源

Immobilization of enzymes and proteins on activated supports permits the simplification of the reactor design and may be used to improve some enzyme properties. In this sense, supports containing epoxy groups seem to be useful to generate very intense multipoint covalent attachment with different nucleophiles placed on the surface of enzyme molecules ( e. g., amino, thiol, hydroxyl groups). However, the intermolecular reaction between epoxy groups and soluble enzymes is extremely slow. To solve this problem, we have designed tailor-made'' heterofunctional epoxy supports. Using these, immobilization of enzymes is performed via a two-step process: ( i) an initial physical or chemical intermolecular interaction of the enzyme surface with the new functional groups introduced on the support surface and ( ii) a subsequent intense intramolecular multipoint covalent reaction between the nucleophiles of the already immobilized enzyme and the epoxy groups of the supports. The first immobilization may involve different enzyme regions, which will be further rigidified by multipoint covalent attachment. The design of some heterofunctional epoxy supports and the performance of the immobilization protocols are described here. The whole protocol to have an immobilized and stabilized enzyme could take from 3 days to 1 week.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据