期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 58, 期 6, 页码 1381-1396出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erl304
关键词
calcium; heavy metal stress; Hordeum vulgare; leaf senescence; lysine motif; receptor-like kinase
The Hordeum vulgare cDNA clone HvLysMR1 that encodes a putative receptor-like protein kinase was identified by restriction fragment differential display-polymerase chain reaction (PCR) comparing cDNA populations derived from mRNAs of primary leaves stressed with chromium for 48 h with controls. The full-length sequence codes for a protein with 622 amino acids which includes characteristic domains of lysine motif receptor-like kinases: an N-terminal signal peptide, two lysine motifs, a transmembrane region, and a serine/threonine kinase domain at the C-terminal end. The expression of HvLYSMR1 is induced during exposure to different heavy metals and its transcript accumulates during leaf senescence. Addition of the calcium ionophore A23187 induces HvLysMR1 expression, indicating the involvement of Ca2+ in the regulation of HvLysMR1. In vitro phosphorylation of HvLysMR1 was analysed with [P-32]ATP. Using the overexpressed and purified HvLysMR1-kinase domain, the phosphorylation of HvLysMR1 could be confirmed by nano-liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) with neutral loss-triggered MS-MS-MS spectra at amino acids localized at the juxtamembrane region. The involvement of HvLysMR1 during heavy metal stress and leaf senescence is discussed.
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