期刊
THERIOGENOLOGY
卷 67, 期 1, 页码 127-133出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2006.09.034
关键词
gene transfer; viral vectors; sperm-mediated gene transfer; gene targeting; artificial chromosome; animal biotechnology
Considerable progress has been made in methods for production of transgenic livestock; beginning with pronuclear microinjection over 20 years ago. New methods, including the use of viral vectors, sperm-mediated gene transfer and somatic cell cloning, have overcome many of the limitations of pronuclear microinjection. It is now possible to not only readily make simple insertional genetic modifications, but also to accomplish, more complex, homozygous gene targeting and artificial chromosome transfer in livestock. (c) 2006 Elsevier Inc. All rights reserved.
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