期刊
NATURE PROTOCOLS
卷 2, 期 3, 页码 661-669出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2007.91
关键词
-
资金
- NCRR NIH HHS [P41RR01395] Funding Source: Medline
- NIGMS NIH HHS [R44 GM069194] Funding Source: Medline
- Wellcome Trust [058551, 068012] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR001395] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R44GM069194] Funding Source: NIH RePORTER
Small d.c. electrical signals have been detected in many biological systems and often serve important functions in cells and organs. For example, we have recently found that they play a far more important role in directing cell migration in wound healing than previously thought. Here, we describe the manufacture and use of a simplified ultrasensitive vibrating probe system for measuring extracellular electrical currents. This vibrating probe is an insulated, sharpened metal wire with a small platinum-black tip (10-30 mu m), which can detect ionic currents in the mu A cm(-2) range in physiological saline. The probe is vibrated at about 300 Hz by a piezoelectric bender. In the presence of an ionic current, the probe detects a voltage difference between the extremes of its movement. The basic, low-cost system we describe is readily adaptable to most laboratories interested in measuring physiological electric currents associated with wounds, developing embryos and other biological systems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据