期刊
JOURNAL OF APPLIED CRYSTALLOGRAPHY
卷 40, 期 -, 页码 539-545出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0021889807012149
关键词
-
资金
- NIGMS NIH HHS [R41 GM073278] Funding Source: Medline
High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results. While mounting of crystal pins, data collection and structure solution are highly automated, crystal harvesting and cryocooling remain formidable challenges towards full automation. To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 mm from a variety of 96-well plates. The UMR is equipped with a versatile tool exchanger providing full operational flexibility. Trypsin crystals harvested and cryoquenched using the UMR have yielded a 1.5 angstrom structure demonstrating the feasibility of robotic protein crystal harvesting.
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