Laminar flow attenuates interferon-induced inflammatory responses in endothelial cells

Objective: Atherosclerosis is a chronic disease that involves inflammation, in which cytokines, including interferon-gamma (IFN-gamma), participate. Endothelial cells (ECs) exposed to IFN-gamma increase the expression of CXC chemokines. ECs subjected to laminar flow (LF) are atheroprotective, despite an unclear mechanism. This study was conducted to analyze whether ECs under LF were protected from IFN gamma-induced responses. Methods: IFN-gamma-treated human umbilical cord ECs were subjected to LF in a well-defined flow chamber system. IFN gamma-induced STAT1 activation and downstream target genes were examined. Results: ECs exposed to IFN-gamma triggered STAT1 activation via the phosphorylation of Tyr701 and Ser727 in STAT1. ECs exposed to LF alone did not activate STAT1. LF exposure of IFN gamma-treated ECs significantly attenuated IFN gamma-induced Tyr701 phosphorylation in a shear-force- and time-dependent manner, whereas Ser727 phosphorylation was unaffected. Consistently, LF inhibited IFN-gamma-induced STAT1 binding to DNA. ECs treated with IFN-gamma induced the expression of three T-cell-specific CXC chemokines (CXCL9, CXCL10 and CXCL11) as well as CIITA, a transcriptional regulator of major histocompatibility complex class II (MHCII). Consistently, LF exposure of IFN gamma-treated ECs reduced the expression of CXC chemokines and CIITA. Conclusions: LF attenuates IFN gamma-induced responses via the suppression of STAT1 activation. Inhibition by LF of the interferon-induced ECs' response may explain some aspects of LF's atheroprotective effects on the endothelium. (C) 2007 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.