期刊
GENES & DEVELOPMENT
卷 21, 期 5, 页码 537-551出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1523007
关键词
Myc; little imaginal discs; trithorax group (TrxG); JmjC; Drosophila; histone demethylase
资金
- NCI NIH HHS [R37 CA057138, R01 CA057138, CA57138] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R37CA057138, R01CA057138] Funding Source: NIH RePORTER
The Myc oncoprotein is a potent inducer of cell growth, cell cycle progression, and apoptosis. While many direct Myc target genes have been identified, the molecular determinants of Myc's transcriptional specificity remain elusive. We have carried out a genetic screen in Drosophila and identified the Trithorax group protein Little imaginal discs ( Lid) as a regulator of dMyc- induced cell growth. Lid binds to dMyc and is required for dMyc- induced expression of the growth regulatory gene Nop60B. The mammalian Lid orthologs, Rbp- 2 (JARID1A) and Plu-1 (JARID1B), also bind to c-Myc, indicating that Lid-Myc function is conserved. We demonstrate that Lid is a JmjC-dependent trimethyl H3K4 demethylase in vivo and that this enzymatic activity is negatively regulated by dMyc, which binds to Lid's JmjC domain. Because Myc binding is associated with high levels of trimethylated H3K4, we propose that the Lid - dMyc complex facilitates Myc binding to, or maintenance of, this chromatin context.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据