期刊
LAB ON A CHIP
卷 7, 期 7, 页码 923-926出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b702548c
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资金
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB005197] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM065293] Funding Source: NIH RePORTER
- NIBIB NIH HHS [EB 005197] Funding Source: Medline
- NIGMS NIH HHS [GM 065293] Funding Source: Medline
Thermoset polyester ( TPE) microfluidic devices were previously developed as an alternative to poly( dimethylsiloxane) ( PDMS) devices, fabricated similarly by replica molding, yet offering stable surface properties and good chemical compatibility with some organics that are incompatible with PDMS. This paper describes a number of improvements in the fabrication of TPE chips. Specifically, we describe methods to form TPE devices with a thin bottom layer for use with high numerical aperture ( NA) objectives for sensitive fluorescence detection and optical manipulation. We also describe plasma- bonding of TPE to glass to create hybrid TPE - glass devices. We further present a simple master- pretreatment method to replace our original technique that required the use of specialized equipment.
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