Simultaneous determination of abacavir, efavirenz and valganciclovir in human serum samples by isocratic HPLC-DAD detection

A rapid, sensitive, and specific reverse phase high performance liquid chromatography with diode array detection procedure for the simultaneous determination of abacavir, efavirenz and valganciclovir in spiked human serum is described. Separation was performed on a 5 Pm Waters Spherisorb column (250 x 4.6 mm ID) with acetonitrile: methanol:KH2PO4 (at pH 5.00) (40:20:40 v/v/v) isocratic elution at a flow rate of 1.0 mL min(-1). Calibration curves were constructed in the range of 50-30,000 ng mL(-1) for abacavir and efavirenz, and 1030,000 ngmL(-1) for valganciclovir in serum samples. The limit of detection and limit of quantification concentrations of the HPLC method were 3.80 and 12.68 ng mL(-1) for obacavir, 2.61 and 8.69 ng mL(-1) for efavirenz, 1.30 and 4.32 ng mL(-1) for valganciclovir. The method has been applied, without any interference from excipients or endogenous substances, for the simultaneous determination of these three compounds in human serum.