Introduction Consequences of lung recruitment with prolonged high positive end-expiratory pressure ( PEEP) ventilation for liver function are unclear. We therefore investigated liver dysfunction during two different ventilation treatment regimens of experimental acute respiratory distress syndrome. Methods Sixteen anaesthetised juvenile pietrain pigs were ventilated in the pressure-controlled mode ( PCV) with an inspiratory fraction of oxygen ( FiO(2)) of 1.0, a respiratory frequency of 30 per minute, a tidal volume of 6 ml/kg, and a PEEP of 5 cm H2O. After lung injury was induced by repeated pulmonary lavage with normal saline, animals were randomly assigned into two groups ( n = 8 each) for a 24-hour trial: PCV ( unchanged ventilation) and PCV with recruitment ( PCV+R) ( starting with a sustained inflation of 50 cm H2O for 1 minute, the ventilation was continued while increasing PEEP in increments of 3 cm H2O every 15 minutes as long as arterial oxygen tension [ PaO2] improved). After recruitment, FiO(2) was reduced to 0.4 and the PEEP was lowered every 15 minutes until PaO2 decreased to 12.0 to 14.7 kPa ( 90 to 110 torr). Serum levels of hyaluronic acid ( HA), routine liver serum markers, and plasma disappearance rate of indocyanine green (ICG) were tested before and after lung injury, and 6 and 18 hours after randomisation. Liver serum markers were also tested at 24 hours. Paraffin sections of liver tissue stained by haematoxylin and eosin were made after euthanisation. Results The PCV+R group exhibited more polymorphonuclear neutrophils and lymphocytes in the liver sinusoids: median score ( interquartile range) of 1.5 ( 1.4 to 1.5) compared to 0.9 ( 0.7 to 1.1) ( p = 0.01). Elevation of bilirubin, aspartate aminotransferase, and lactate dehydrogenase was more prominent in the PCV+R group. Plasma disappearance rate of ICG indicated no liver dysfunction. HA levels in the PCV+R group gradually increased and were significantly higher ( p < 0.001) at 6 and 18 hours with 59 ( 57 to 64) and 75 ( 66 to 84) ng/ml, respectively, than in the PCV group with 34 ( 32 to 48) and 41 ( 38 to 42) ng/ml, respectively. Conclusion The PCV+R group showed a more prominent inflammatory reaction in their liver sinusoids accompanied by increased serum levels of liver enzymes and HA. Therefore, recruitment with higher PEEP levels for treatment of respiratory failure might lead to liver dysfunction.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据