期刊
JOURNAL OF SURGICAL RESEARCH
卷 137, 期 1, 页码 38-45出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2006.05.043
关键词
myenteric glia; interleukin; matrix metalloproteinase; secretion; immunomodulation
类别
资金
- NIDDK NIH HHS [T32 DK07698-09, 5R01 DK41204-14] Funding Source: Medline
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK041204, T32DK007698] Funding Source: NIH RePORTER
Western blotting of culture media of myenteric glia stained positive for GFAP revealed increased secretion of matrix metalloproteinase-9 (MMP-9) in interleukin-1 beta (IL-1 beta) stimulated cells (10 ng/mL) versus control (142 +/- 19 versus 42 +/- 19, P < 0.05). Interleukin-6 (IL-6) stimulated cells also showed increased expression of MMP-9 (10 ng/mL) versus control (69 +/- 14 versus 4 +/- 2, P < 0.01). Control and cytokine-stimulated cells secreted MMP-2 constituitively. Gelatin zymography demonstrated that products were biologically active. Cytoplasmic staining for MMP-9 was detected in IL-1 beta and IL-6-stimulated cells but was negligible in controls. Cultured myenteric glia are responsive to IL-1 beta and IL-6 stimulation by secreting MMPs into the extracellular environment. (c) 2007 Elsevier Inc. All rights reserved.
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