期刊
TOXICOLOGICAL SCIENCES
卷 95, 期 1, 页码 249-256出版社
OXFORD UNIV PRESS
DOI: 10.1093/toxsci/kfl141
关键词
spermatogenesis; p-nonylphenol; cleaved caspase-3; stages; TUNEL
类别
The cumulative effects of environmental toxicants, for example, the alkylphenol, para-nonylphenol (p-NP) are of concern. Our previous study showed that p-NP reduced several testicular morphometric parameters, including sperm count. The present study reexamined material collected in that study to determine the mechanistic basis of p-NP action on spermatogenic development in the offspring. Seven-day pregnant Sprague-Dawley rats were treated with vehicle or 100 or 250 mg/kg p-NP through gestation, lactation and afterward directly to all male offspring until 10 weeks of age. Both doses of p-NP significantly (p < 0.02) increased the number of germ cells with in-situ end-labeled fragmented DNA (TUNEL positive) by 1.9-fold and 1.7-fold, respectively, and specifically in stages XII-XIV and I-III. TUNEL-labeling was, however, selective, and excluded labeling of basel cells with apoptotic morphology. Cleaved caspase-3 immunohistochemistry strongly labeled basel cells (spermatogonia and early spermatocytes) with condensed marginated chromatin but not degenerate germ cells lacking definitive nuclear material found throughout the epithelium. Only the caspase index (ratio of number of caspase positive to number of degenerate cells of the 100-mg/kg p-NP group was significantly (p < 0.05) threefold greater than controls. Whereas both doses and either 250 or 100 mg/kg treatment alone significantly (p < 0.002) increased the frequencies of stages IV-VI and all stages containing late-stage spermatogenic (XII-XIII) and meiotic cell divisions (XIV). Thus, p-NP, and environmentally persistent xenoestrogen, insidiously alters the spermatalogic cycle and spermatogenic process in male offspring.
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