期刊
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
卷 26, 期 10-12, 页码 1635-1639出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15257770701549061
关键词
single nucleotide polymorphism; mismatch base pairs; fluorescence
Addition of mercury (II) cation to fluorescent-labeled duplex involving a T:T mismatch base pair and silver (I) cation to fluorescent-labeled duplex involving a C:C mismatch base pair significantly changed the fluorescence intensity, but no significant change in the fluorescence intensity was observed for duplexes involving the other base pairs. The fluorescence spectral change upon addition of the metal cation can discriminate T:T and C:C mismatch base pairs from the other base pairs. Our results certainly support the idea that the fluorescence spectral change upon addition of the metal cation could be a convenient strategy for the mismatch base pair detection by the heteroduplex analysis, and may eventually Lead to progress in single nucleotide polymorphism genotyping.
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