QTL for fatty acid composition of maize kernel oil in Illinois high oil x B73 backcross-derived lines

Maize (Zea mays L.) produces high-quality oil valued for oxidative stability and low concentrations of saturated fatty acids. The nutritional value of maize oil could be improved by increasing the concentration of oleic acid, a heart-friendly monounsaturated fatty acid. To identify quantitative trait loci (QTL) for the major fatty acids constituting oil from maize kernels, we produced 150 backcross1-derived S-1 (BC1S1) lines from donor parent, Illinois High Oil (IHO), and recurrent parent, B73. There was a positive phenotypic correlation between oil and oleic acid (r(p) = 0.47**, alpha <= 0.01) and negative correlations between oil and linoleic acid (r(p) = -0.46**), and between oleic and linoleic acids (r(p) = -0.99**). Multiple regression models with QTL detected by composite interval mapping (CIM) on a genetic map with length = 1486 cM, explained 15.4, 41.6, 51.0, 59.6, and 47.9% of the phenotypic variation for palmitic, stearic, oleic, linoleic, and linolenic acids, respectively. A 6-cM interval on chromosome 6 (bin 6.04) includes QTL for stearic, oleic, linoleic, and linolenic acids, explains 10.9 to 39.6% of the variation for these fatty acids, and is 10 to 16 cM from QTL for oil. Another region on chromosome 6 (bin 6.01) includes QTL for oleic and linoleic acids and was epistatic with the QTL in bin 6.04. One or both of these two QTL regions on chromosome 6 may be responsible for fatty acid variation previously attributed to linoleic acid1.