期刊
ANIMAL
卷 2, 期 1, 页码 100-104出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1751731107000961
关键词
ferrous ascorbate; lipid peroxidation; reactive oxygen species; sperm motility; viability
Numerous factors influence male fertility, one of these being the oxidative stress, which has elicited enormous interest recently. In sperm, induction of oxidation decreases motility and viability but increases lipid peroxidation (00). The optimum dose of ferrous ascorbate (FeAA: FeSO(4) + ascorbic acid) for inducing oxidative stress by affecting motility, viability and LPO has been ascertained in local crossbred cattle bull spermatozoa. The fractions of spermatozoa suspended in 2.9% sodium citrate were subjected to three doses of FeAA (100: 500, 150: 750, 200: 1000; mu mol/l FeSO(4): mu mol/l ascorbic acid). These fractions were assessed for various parameters. Increase in the incubation period and promoter concentration induced a decrease in motility and viability, but an increase in LPO. Among three doses of FeAA 150: 750 mu mol/l ascorbic acid is suggested to be the optimum/best dose as it induces the oxidative stress/LPO to a significant extent and also maintains better motility and viability as compared with the other two doses, and such conditions may enhance the fertilising potential of bull spermatozoa.
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