期刊
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
卷 83, 期 1, 页码 41-46出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/jctb.1774
关键词
immobilization; glucose oxidase; poly(vinyl alcohol); sol-gel; hybrid
BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chernically-linked PVA, freeze-thawed PVA cryogel, tetramethoxysilane (TMOS) sol-gel-PVA hybrid material, and alumina sol-gel-PVA hybrid material. RESULTS: Overall, the membranes showed good sensitivity except for the chemically cross-linked PVA. However, the main differences with the enzyme immobilization methods were enzyme leakage and values of K-m(app). CONCLUSION: Freeze-thawed PVA-GOD membranes and TMOS-PVA, which showed satisfactory sensitivity and adequate value of K-m(app), were quite promising as support materials for immobilizing GOD. (c) 2007 Society of Chemical Industry.
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