期刊
CONSERVATION GENETICS
卷 9, 期 5, 页码 1327-1331出版社
SPRINGER
DOI: 10.1007/s10592-007-9476-y
关键词
expressed sequence tag; germplasm; microsatellite; SSRs; tea plant
资金
- Hi-Tech Research and Development Program of China (863 Plan) [2006AA10Z171]
- Qianjiang Personnel Program of Zhejiang Province [2006R10042]
- National Key Technologies RD Program [2006BAD13B06-1]
- R&D Infrastructure and Facility Development Program [2005DKA21002-08]
- NSF of Zhejiang [Y305124, Y305105]
A total of 31 expressed sequence tag (EST)derived polymorphic microsatellites from tea plant, Camellia sinensis (L.) O. Kuntze, were generated and characterized using the ESTs of the author's EST sequencing project and other sources. A set of 40 accessions tea germplasms had been used to examine the diversity. Among the 31 microsatellite loci, 24 had two to eight polymorphic alleles. Observed heterozygosity (H,,) were relatively higher (on an average of 0.533), varying from 0.175 (primer 21) to 0.950 (primer 228). Cross-species polymorphic amplification in other four species and two varieties of section Thea (L.) Dyer genus Camellia L. was successful for the 24 loci. Contribution of the 24 novel EST-SSR primers presented here will provide necessary and powerful molecular tools for management and conservation studies on the tea germplasms in the future.
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