期刊
SPINAL CORD
卷 46, 期 1, 页码 39-44出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.sc.3102057
关键词
inosine; contusion injury; macrophage/microglia; immunomodulatory; secondary damage
Study design: In vivo study using a moderate spinal cord contusion injury (SCI) model in adult rat. Objective: To assess the immunomodulatory effects of the purine nucleoside inosine on macrophage/microglia activation at and near the lesion site and in white matter areas remote from the injury epicenter. Setting: Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY, USA. Methods: Animals (N = 56) were injured using a moderate SCI at T9-T10 spinal level and were divided into three groups, depending on treatment paradigm. Rats received either intraperitoneal or subcutaneous injections of inosine ( N 28) or vehicle ( N 28). Spinal cord tissue was processed for ED-1 immunoreactivity and the volume fraction of ED-1(+) profiles was calculated using the Cavalieri method and unbiased stereology. Results: The volume fraction of ED-1(+) profiles within gray and lateral white matter regions at and around the lesion site was significantly reduced only following a twice daily-6 week treatment course, compared with vehicle controls, and white matter areas remote from the lesion were unaffected by all inosine treatment paradigms. Conclusions: Continued subcutaneous delivery of inosine, beginning 15-min post-SCI and persisting throughout the survival period of 6 weeks exerted immunomodulatory effects at and around the lesion site.
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