期刊
CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES
卷 36, 期 5, 页码 566-574出版社
CANADIAN JOURNAL NEUROLOGICAL SCIENCES INC
DOI: 10.1017/S0317167100008052
关键词
-
资金
- Department of Pathology and Laboratory Medicine, University of Calgary Facility of Medicine and Alberta Children's Hospital
- Calgary Laboratory Services
- Calgary Health Region, Province of Alberta
Background. We studied alpha-B-crystallin, a small heat shock chaperone protein upregulated by various stresses, as an immunocytochemical tissue marker of epileptic foci. Methods: We examined 45 resected brain tissues of epileptic patients, 16 months to 23 years. Postmortem brains of 2 epileptic children and 20 normal fetuses and neonates of 10-41 weeks gestation similarly were studied. Immunocytochemical demonstration of cc-B-crystallin was supplemented by neuronal, glial and inflammatory cell markers and electron microscopy (EM) in surgical cases. Autopsy brain tissue of children without epilepsy or neurological disease served as controls. Results: In all resections, alpha-B-crystallin was overexpressed in astrocytes and oligodendrocytes, including satellite cells adherent to neurons, and occasionally in neurons of neocortex, hippocampus and amygdala. In six cases, reactivity was most intense at or near the epileptic focus, with a diminishing gradient of intensity for 2-3 cm; similar focal expression was seen in autopsy cases. Presence or absence of histological structural lesions was independent of alpha-B-crystallin expression. Balloon cells and giant atypical cells in tuberous sclerosis were intensely reactive. Reactivity was present in DNETs. No correlation occurred with microglial activation, inflammation or gliosis; no ultrastructural alterations were seen. No expression was seen in fetal brains at any age. Conclusions: Immunoreactive alpha-B-crystallin is a reliable tissue marker of epileptic foci, regardless of presence or absence of structural lesions; at times it maps the extent of a focus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据