期刊
ANALYTICAL METHODS
卷 7, 期 14, 页码 6027-6033出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay01576f
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资金
- National Science Foundation [CBET:1159829]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1159829] Funding Source: National Science Foundation
We describe an aminooxy reagent for the capture of trace aldehyde and ketone volatile organic compounds (VOCs) in exhaled breath. The reagent, 4-(2-aminooxyethyl)-morpholin-4-ium chloride (AMAH), when coated onto micropillars within a silicon microreactor, chemoselectively and covalently retains carbonyl VOCs from exhaled breath. The AMAH-carbonyl adducts are then eluted from the microreactor with methanol and directly analyzed by Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometry (MS), where the aminium ion of the reagent enhances the sensitivity for high mass accuracy. We also outline a protocol for treatment of the AMAH-carbonyl adducts with poly(4-vinylpyridine) to afford the corresponding volatile carbonyl adducts that now can be analyzed by gas chromatography-mass spectrometry (GC-MS). This convenient protocol imparts flexibility for the identification and quantification of isomeric VOCs using both FT-ICR-MS and GC-MS. Representative breath analyses are given to illustrate this applicability of AMAH.
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