期刊
CANADIAN JOURNAL OF MICROBIOLOGY
卷 56, 期 5, 页码 440-444出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/W10-021
关键词
methanogens; biogas plant; quantitative PCR; PCR-RFLP
资金
- German Federal Ministry of Food, Agriculture, and Consumer Protection
- Agency of Renewable Resources [22011804]
- German Federal Ministry of Education and Research (BMBF) [03SF0317M]
Energy production of renewable raw material is of increasing importance for sustainable energy production. As an indispensable prerequirement for further upgrading of technical equipment and operation modes of biogas plants, a deeper knowledge of the microbial community responsible for methane formation is crucial. To overcome these limitations a mesophilic biogas plant converting pig manure. maize silage, and grains of crop was sampled and subsequently analysed by construction of a methanogenic Archaea specific 16S rRNA gene clone library combined with PCR-RFLP analysis and group-specific quantitative real-time PCR (qPCR). Seventy percent of all analysed clones belonged to the order Methanomicrobiales, whereas 13% belonged to Methanosarcinales, 6% belonged to the Methanobacteriales group, and 11% of all detected clones were assigned to the CA 11 and Arch1 cluster. Comparable percentages were obtained with qPCR: 84% of all detected 16S rRNA gene copy numbers were affiliated with the Methanomicrobiales, while only 14% belonged to the Methanosarcinales and 2% to the Methanobacteriales order. In conclusion, both approaches detected similar archaeal groups and revealed nearly the same abundance, pointing to a predominance of hydrogenotrophic methanogens in the biogas plant.
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