期刊
CANADIAN JOURNAL OF MICROBIOLOGY
卷 54, 期 9, 页码 718-724出版社
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
DOI: 10.1139/W08-060
关键词
biofilm; CSH; genotype; Candida albicans; Candida dubliniensis
资金
- Marie Curie RTN program [MRTN-CT-2004-512481]
- Science and Technology Assistance Agency [APVT-20-01590]
- MVTS [6RP/MRTN/CT/2004/512481]
- Slovak Grant Agency of Science [VEGA 1/4324/07]
- Comenius University [UK/135/2005]
Candida infections are frequently associated with formation of biofilms on artificial medical devices. This work studied variation of cell surface hydrophobicity (CSH) and formation of biofilm in relation to Candida albicans and Candida dubliniensis genotypes and an effect of some conventional antifungal agents on both CSH and biofilm. The 50 isolates of C. albicans and C. dubliniensis were classified into genotypes A, B, C, and D, genotype D being exclusively represented by C. dubliniensis. No significant differences between CSH of genotypes A and B and B and C were observed with respect to cultivation temperature 25 or 37 degrees C. Candida dubliniensis showed increased CSH in comparison with other C. albicans genotypes (pa < 0.001) regardless of temperature used. Using XTT reduction assay and dry masses, genotypes B and C showed reduced ability to form biofilm in comparison with genotype A (pa < 0.05) and C. dubliniensis (pa < 0.001). Fluconazole reduced biofilm in C. albicans genotypes A, B, and C (pa < 0.05) but not CSH. The opposite effect was observed in C. dubliniensis. Voriconazole effectively reduced both biofilm formation and CSH in all tested genotypes of C. albicans and C. dubliniensis (pa < 0.05).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据