Agents blocking the nuclear factor-kappa B pathway are effective inhibitors of endometriosis in an in vivo experimental model

Background: In vitro studies suggest that the transcription factor nuclear factor-kappa B (NF-kappa B) is implicated in the transduction of proinflammatory signals in endometriosis. The aim of this study was to investigate the involvement of NF-kappa B and the processes regulated by NF-kappa B in the initial development of endometriotic lesions in vivo. Methods: Endometriosis was induced in nude mice by intraperitoneal injection of fluorescent-labeled menstrual endometrium. Two NF-kappa B inhibitors (BAY 11-7085 and SN-50) were injected intraperitoneally on days 0, 2 and 4 after endometriosis induction, and endometriotic lesions were recovered on day 5. Number, mass, fluorimetry and surface (morphometry) of endometriotic lesions were quantified. NF-kappa B activation, intercellular adhesion molecule (ICAM)-1 expression, cell proliferation and apoptosis were evaluated by immunohistochemical analyses and the TUNEL method. Results: Both NF-kappa B inhibitors induced a significant reduction in lesion development compared to control mice. NF-kappa B activation and ICAM-1 expression of endometriotic lesions were significantly reduced in treated mice, and cell proliferation was significantly reduced in BAY 11-7085-treated mice. Both inhibitors produced a significant increase in apoptosis of endometriotic lesions, as assessed by active caspase-3 immunostaining and the TUNEL method. Conclusion: This study demonstrates, for the first time, that the NF-kappa B pathway is implicated in the development of endometriotic lesions in vivo and that NF-kappa B inhibition reduces ICAM-1 expression and cell proliferation, but increases apoptosis of endometriotic lesions, diminishing the initial development of endometriosis in an animal model. Copyright (C) 2007 S. Karger AG, Basel.