期刊
NEONATOLOGY
卷 93, 期 1, 页码 19-27出版社
KARGER
DOI: 10.1159/000105521
关键词
developmental biology; fetal programming; superoxide; vascular endothelium
类别
资金
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [T32HD041922, K08HD050359] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL062483, R56HL062483, K02HL004495, R01HL081750] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R21ES012268] Funding Source: NIH RePORTER
- NHLBI NIH HHS [R01 HL081750-03, HL-62483, R01 HL081750-01, R01 HL081750-02, R01 HL081750-04, R01 HL081750, HL-04495] Funding Source: Medline
- NICHD NIH HHS [K08 HD050359, HD-050359, HD-41922] Funding Source: Medline
- NIEHS NIH HHS [ES-012268] Funding Source: Medline
Background: Animal models have demonstrated that maternal undernutrition or early gestation glucocorticoid exposure induces endothelial dysfunction in the offspring. Objectives: We sought to determine whether early gestation dexamethasone (DEX) exposure is further associated with increased vascular superoxide anion production. Methods: DEX (0.28 mg/kg/day i.v. for 48 h) was administered to pregnant ewes at 27-28 days' gestation (term 145 days). Tissues were harvested from DEX-exposed and control lambs at 125 days' gestation (n = 6 for each group) and 4 months following delivery (n = 9 and 12, respectively). Results: By lucigenin-enhanced chemiluminescence, coronary and mesenteric arteries from DEX-exposed fetuses exhibited diminished basal superoxide production (both p < 0.01). Similarly, DEX-exposed carotid arteries from 4-month-old lambs had decreased superoxide production (p < 0.01) that localized to the endothelium by endothelial cell culture and dihydroethidium fluorescence. In contrast, DEX-exposed coronary arteries from the 4-month-old sheep had increased superoxide production (p < 0.05). Although early gestation DEX exposure did not alter lipid peroxidation, DEX exposure was associated with significantly increased renal and cerebral cortex aconitase activity (consistent with decreased protein oxidation). These changes occurred in the absence of alterations in renal cortex superoxide dismutase activity. Conclusions: We conclude that in a DEX-exposure model of fetal programming, endothelial superoxide production and protein oxidation are decreased in the mesenteric and carotid circulation. This contrasts with the postnatal coronary artery-specific increase in superoxide production. Copyright (c) 2007 S. Karger AG, Basel.
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