Determination of cytotoxicity of nephrotoxins on murine and human kidney cell lines

The present study investigates renal inner medullary collecting duct (mIMCD3) cells and human embryonic kidney cells (HEK293) for evaluation of cytotoxicity of nephrotoxic compounds. The 24 h LC50 values for cisplatin, paraquat and ibuprofen in mIMCD3 cells were 135, 155 and 3600 mu M, respectively. The 24 h LC50 values for paraquat and ibuprofen in HEK293 cells were 180 and 1000 mu M, respectively. Effects of hyperosmolality on cytotoxicity of paraquat were additive in mIMCD3 cells. These data demonstrate that renal hyperosmolality has an additive effect on cytoxicity of paraquat.