期刊
CALCIFIED TISSUE INTERNATIONAL
卷 103, 期 6, 页码 675-685出版社
SPRINGER
DOI: 10.1007/s00223-018-0463-8
关键词
Osteocyte; Lacuna morphology; Mechanotransduction; Ex vivo mechanical loading
资金
- European Commission through MOVE-AGE, an Erasmus Mundus Joint Doctorate programme [2011-0015]
Hormonal changes during lactation are associated with profound changes in bone cell biology, such as osteocytic osteolysis, resulting in larger lacunae. Larger lacuna shape theoretically enhances the transmission of mechanical signals to osteocytes. We aimed to provide experimental evidence supporting this theory by comparing the mechanoresponse of osteocytes in the bone of lactating mice, which have enlarged lacunae due to osteocytic osteolysis, with the response of osteocytes in bone from age-matched virgin mice. The osteocyte mechanoresponse was measured in excised fibulae that were cultured in hormone-free medium for 24h and cyclically loaded for 10min (sinusoidal compressive load, 3000 mu epsilon, 5Hz) by quantifying loading-related changes in Sost mRNA expression (qPCR) and sclerostin and -catenin protein expression (immunohistochemistry). Loading decreased Sost expression by similar to threefold in fibulae of lactating mice. The loading-induced decrease in sclerostin protein expression by osteocytes was larger in lactating mice (55% decrease +/- 14 (+/- SD), n=8) than virgin mice (33% decrease +/- 15, n=7). Mechanical loading upregulated -catenin expression in osteocytes in lactating mice by 3.5-fold (+/- 0.2, n=6) which is significantly (p<0.01) higher than the 1.6-fold increase in -catenin expression by osteocytes in fibulae from virgin mice (+/- 0.12, n=4). These results suggest that osteocytes in fibulae from lactating mice with large lacunae may respond stronger to mechanical loading than those from virgin mice. This could indicate that osteocytes residing in larger lacuna show a stronger response to mechanical loading.
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