期刊
CALCIFIED TISSUE INTERNATIONAL
卷 94, 期 5, 页码 495-509出版社
SPRINGER
DOI: 10.1007/s00223-013-9833-4
关键词
Bone metabolism; Carbonic anhydrase; Quinolinic acid; SaOS-2 cell; Ca-carbonate; Suberites domuncula extract
资金
- ERC Advanced Investigator Grant [268476 BIOSILICA]
- Deutsche Forschungsgemeinschaft [Schr 277/10-3]
- European Commission [604036, 286059, 268476, 311848]
- International Human Frontier Science Program
Ca-phosphate/hydroxyapatite (HA) crystals constitute the mineral matrix of vertebrate bones, while Ca-carbonate is the predominant mineral of many invertebrates, like mollusks. Recent results suggest that CaCO3 is also synthesized during early bone formation. We demonstrate that carbonic anhydrase-driven CaCO3 formation in vitro is activated by organic extracts from the demosponge Suberites domuncula as well as by quinolinic acid, one component isolated from these extracts. Further results revealed that the stimulatory effect of bicarbonate (HCO3 (-)) ions on mineralization of osteoblast-like SaOS-2 cells is strongly enhanced if the cells are exposed to inorganic polyphosphate (polyP), a linear polymer of phosphate linked by energy-rich phosphodiester bonds. The effect of polyP, administered as polyP (Ca2+ salt), on HA formation was found to be amplified by addition of the carbonic anhydrase-activating sponge extract or quinolinic acid. Our results support the assumption that CaCO3 deposits, acting as bio-seeds for Ca-carbonated phosphate formation, are formed as an intermediate during HA mineralization and that the carbonic anhydrase-mediated formation of those deposits is under a positive-negative feedback control by bone alkaline phosphatase-dependent polyP metabolism, offering new targets for therapy of bone diseases/defects.
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