期刊
CALCIFIED TISSUE INTERNATIONAL
卷 92, 期 3, 页码 296-306出版社
SPRINGER
DOI: 10.1007/s00223-012-9685-3
关键词
Bone marrow transplantation; Bone marrow-derived cell; Bone healing; Bone fracture; GFP
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [20791515, 22791766, 24592766, 24659891]
- Grants-in-Aid for Scientific Research [24659891, 22791766, 20791515, 24592766, 22791977] Funding Source: KAKEN
Bone healing is a complex and multistep process in which the origin of the cells participating in bone repair is still unknown. The involvement of bone marrow-derived cells in tissue repair has been the subject of recent studies. In the present study, bone marrow-derived cells in bone healing were traced using the GFP bone marrow transplantation model. Bone marrow cells from C57BL/6-Tg (CAG-EGFP) were transplanted into C57BL/6 J wild mice. After transplantation, bone injury was created using a 1.0-mm drill. Bone healing was histologically assessed at 3, 7, 14, and 28 postoperative days. Immunohistochemistry for GFP; double-fluorescent immunohistochemistry for GFP-F4/80, GFP-CD34, and GFP-osteocalcin; and double-staining for GFP and tartrate-resistant acid phosphatase were performed. Bone marrow transplantation successfully replaced the hematopoietic cells into GFP-positive donor cells. Immunohistochemical analyses revealed that osteoblasts or osteocytes in the repair stage were GFP-negative, whereas osteoclasts in the repair and remodeling stages and hematopoietic cells were GFP-positive. The results indicated that bone marrow-derived cells might not differentiate into osteoblasts. The role of bone marrow-derived cells might be limited to adjustment of the microenvironment by differentiating into inflammatory cells, osteoclasts, or endothelial cells in immature blood vessels.
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