期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 28, 期 1, 页码 20-29出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.01122-07
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资金
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES013773] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM031819, F32GM077900, R01GM061645] Funding Source: NIH RePORTER
- NIEHS NIH HHS [R01 ES013773, ES 013773] Funding Source: Medline
- NIGMS NIH HHS [GM077900-01, F32 GM077900, R01 GM061645, GM31819, GM61645, R01 GM031819] Funding Source: Medline
- PHS HHS [5T32GMOO 10330] Funding Source: Medline
The Kluyveromyces lactis ter1-16T strain contains mutant telomeres that are poorly bound by Rap1, resulting in a telomere-uncapping phenotype and significant elongation of the telomeric DNA. The elongated telomeres of ter1-16T allowed the isolation and examination of native yeast telomeric DNA by electron microscopy. In the telomeric DNA isolated from ter1-16T, looped molecules were observed with the physical characteristics of telomere loops (t-loops) previously described in mammalian and plant cells. ter1-16T cells were also found to contain free circular telomeric DNA molecules (t-circles) ranging up to the size of an entire telomere. When the ter1-16T uncapping phenotype was repressed by overexpression of RAP1 or recombination was inhibited by deletion of rad52, the isolated telomeric DNA contained significantly fewer t-loops and t-circles. These results suggest that disruption of Rap1 results in elevated recombination at telomeres, leading to increased strand invasion of the 3' overhang within t-loop junctions and resolution of the t-loop junctions into free t-circles.
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