期刊
PLANT PHYSIOLOGY
卷 146, 期 1, 页码 310-320出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.107.106831
关键词
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The beta-substituted alanine (Ala) synthase (Bsas) family in the large superfamily of pyridoxal 5'-phosphate-dependent enzymes comprises cysteine (Cys) synthase (CSase) [O-acetyl-serine (thiol) lyase] and beta-cyano-Ala synthase (CASase) in plants. Nine genomic sequences encode putative Bsas proteins in Arabidopsis thaliana. The physiological roles of these Bsas isoforms in vivo were investigated by the characterization of T-DNA insertion mutants. Analyses of gene expression, activities of CSase and CASase, and levels of Cys and glutathione in the bsas mutants indicated that cytosolic Bsas1; 1, plastidic Bsas2; 1, and mitochondrial Bsas2; 2 play major roles in Cys biosynthesis. Cytosolic Bsas1; 1 has the most dominant contribution both in leaf and root, and mitochondrial Bsas2; 2 plays a significant role in root. Mitochondrial Bsas3; 1 is a genuine CASase. Nontargeted metabolome analyses of knockout mutants were carried out by a combination of gas chromatography time-of-flight mass spectrometry and capillary electrophoresis time-of-flight mass spectrometry. The level of gamma-glutamyl-beta-cyano-Ala decreased in the mutant bsas3; 1, indicating the crucial role of Bsas3; 1 in beta-cyano-Ala metabolism in vivo.
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