期刊
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
卷 31, 期 11, 页码 3099-3102出版社
WILEY-V C H VERLAG GMBH
DOI: 10.5012/bkcs.2010.31.11.3099
关键词
Peptide nucleic acid; DNA detection; Ferrocene dendrimer; Label-free; Electrostatic interaction
资金
- Ministry of Education, Science and Technology [2010-0008213, 2010-0015442]
- Korean Government [F01-2008-000-10153-0]
- Myongji University
A label-free DNA detection method was developed for a simple electrochemical DNA sensor with a short assay time Self-assembled monolayers of peptide nucleic acid were used as a probe on gold electrodes The formation of the self-assembled monolayers on the gold electrodes was successfully checked by means of cyclic voltammetry The target DNA, hybridized with peptide nucleic acid, can be detected by the anodic peak current of ferrocene dendrimers, which interact electrostatically with the target DNA This anodic peak current was measured by square wave voltammetry at 0 3 V to decrease the detection limit on the order of the nanomolar concentrations As a result, the label-free electrochemical DNA sensor can detect the target DNA in concentrations ranging from 1 nM to 1 mu M with a detection limit of 1 nM
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