Synthesis and Evaluation of Benzoquinolinone Derivatives as SARS-CoV 3CL Protease Inhibitors

For the discovery of new antivirals against Severe Acute Respiratory Syndrorne-coronavirus (SARS-CoV), we prepared and evaluated several benzoguinoline compounds as its 3C-like protease (3CL-pro) inhibitors. Based oil the computer modeling Study that each of the two rigid benzoquinolinone and N-phenotetrazole moieties of the compound I is bound to the S I and S2 sites, respectively, of the SARS protease by forming H-bonds and hydrophobic interactions, we designed and synthesized alkylated benzoquinolities at both the sites of the hydroxyl groups. We found that the compound 2a showed five times higher inhibiting activity against the 3CLpro compared to the compound 1.