期刊
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
卷 31, 期 2, 页码 435-441出版社
WILEY-V C H VERLAG GMBH
DOI: 10.5012/bkcs.2010.31.02.435
关键词
DNA-binding; Palladium(II) complex; Cytotoxicity; 1,10-Phenanthroline; Dithiocarbamate
资金
- Research Council of University of Sistan and Baluchestan
- University of Tehran
We report the preparation and characterization of a new and water Soluble complex of palladium(II) With 1, 10-phenanthroline and butyldithiocarbamate ligands. This compound has been studied through spectroscopic techniques, H-1 NMR, IR, electronic spectra and elemental analysis and conductivity measurements. The complex shows 50% cytotoxic concentration (Ic(50)) value against chronic myelogenous leukemia cell line, K562, much lower than that of cisplatin. Thus the mode of binding of this complex to calf thymus DNA have been extensively investigated by isothermal titration UV-visible spectrophotometry, fluorescence, gel filteration and other methods. UV-visible Studies show that the complex exhibits cooperative binding with DNA and remarkably denatures the DNA at extremely low concentration (similar to 13 mu M). Fluorescence Studies indicate that the complex intercalate into DNA. Gel filtration Studies suggest that the binding of Pd(II) complex with DNA is strong enough that it does not readily break. In these interaction studies, several thermodynamic and binding parameters are also determined which may reflect the mechanism of action of this type of compound with DNA.
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