期刊
MOLECULAR PHARMACOLOGY
卷 75, 期 1, 页码 151-157出版社
AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
DOI: 10.1124/mol.108.049502
关键词
-
资金
- National Research Program for Genomic Medicine, National Science and Technology Program for Agricultural Biotechnology, National Science Counci
- Committee on Chinese Medicine and Pharmacy
- Department of Health [CCMP 96-RD-201, CCMP 97-RD-201]
- China Medical University, Taiwan
Vanillin has been reported to exhibit anti-invasive and antimetastatic activities by suppressing the enzymatic activity of matrix metalloproteinase-9 (MMP-9). However, the underlying mechanism of anti-invasive activity remains unclear so far. Herein we demonstrate that vanillin reduced 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 gelatinolytic activity and suppressed cell invasion through the down-regulation of MMP-9 gene transcription in HepG2 cells. Vanillin significantly reduced the 6.6-fold invasive capacity of HepG2 cells in noncytotoxic concentrations, and this anti-invasive effect was concentration-dependent in the Matrigel invasion assay. Moreover, vanillin significantly suppressed the TPA-induced enzymatic activity of MMP-9 and decreased the induced mRNA level of MMP-9. Analysis of the transcriptional regulation indicated that vanillin suppressed MMP-9 transcription by inhibiting nuclear factor-kappa B (NF-kappa B) activity. Western blot further confirmed that vanillin inhibited NF-kappa B activity through the inhibition of I kappa B-alpha phosphorylation and degradation. In conclusion, vanillin might be a potent anti-invasive agent that suppresses the MMP-9 enzymatic activity via NF-kappa B signaling pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据