期刊
ANALYTICAL METHODS
卷 7, 期 20, 页码 8961-8966出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay01583a
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资金
- BioPersMed (COMET K) - Federal Ministry of Transport, Innovation and Technology (BMVIT) [825329]
- Federal Ministry of Economics and Labour/the Federal Ministry of Economy, Family and Youth (BMWA/BMWFJ)
- Styrian Business Promotion Agency (SFG)
Establishing accurate and precise analytical methods for vitamin D species is of rising importance as awareness of the vitamin D deficiency problem grows. Here, we report an analytical method for quantifying vitamin D and relevant vitamin D metabolites by high-resolution mass spectrometry (HRMS). We combine a straightforward time saving sample preparation and chromatographic separation, employing gradients and a column-switching technique, with high-resolution MS detection, extending its applicability to a broader range of relevant vitamin D metabolites. We calibrated the method to NIST standards and validated it for 25(OH)D-2 and 25(OH)D-3. We also present short-and long-term sample-stability data and proof-of-concept by applying the method to serum samples from a healthy patient library that were previously measured by immunoassay. Compared to the immunoassay data, our method showed a good correlation of 25(OH)D-3 concentrations. Thus, we show that quantitative HRMS is now within reach of clinical diagnostics for vitamin D and its metabolites.
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