期刊
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
卷 155, 期 1, 页码 40-43出版社
SPRINGER
DOI: 10.1007/s10517-013-2075-7
关键词
neurons; glutamate receptors; nitric oxide; ATP; inhibitors of NO-synthase
资金
- Russian Foundation for Basic Research [05 08-04-01802-a, 11-04-01961-a]
We studied the effects of selective inhibitors of neuronal and inducible NO-synthase (7-nitroindazole and aminoguanidine) and non-selective NO-synthase inhibitor L-NAME on ATP content and survival of cultured rat cerebellar neurons during hyperstimulation of glutamate receptors with toxic doses of glutamate. Application of 100 mu M glutamate reduced ATP content in the primary culture of 7-8- and 14-15-day-old cerebellar granule cells by 66 and 49%, respectively, in comparison with the control. Inhibition of nitric oxide synthesis with 7-nitroindazole during glutamate exposure in the culture of 7-8-day-old neurons and with 7-nitroindazole and aminoguanidine in the culture of 14-15-day-old neurons ensured better protection of cells from ATP level decrease than non-specific inhibition with L-NAME. In addition, inhibition of neuronal and inducible NO-synthase during glutamate exposure decreased death of young neurons, whereas death of old neurons remained high under these conditions.
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