期刊
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
卷 152, 期 2, 页码 219-223出版社
SPRINGER
DOI: 10.1007/s10517-011-1493-7
关键词
L-asparaginase; antitumor enzyme; Yersinia pseudotuberculosis
Recombinant E. coli strain producing Y. pseudotuberculosis Q66CJ2 (YpA) L-asparaginase II was created. Gene ansB homologue encoding Y. pseudotuberculosis IP 32953 L-asparaginase precursor was synthesized. The gene was cloned in pBad24 expression vector and expressed in E. coli BL21 (DE3) strain. Optimal conditions for the producer strain culturing were selected. An effective method for isolation and purification of the enzyme by two-staged column chromatography was developed.
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